RECIPE: Biogenic Amine Standards
Tue, Jan 10 2012 07:46
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NE : Norepinephrine (Arterenol)
DOPAC : 3, 4-dihydroxyphenylacetic acid
DOPAMINE: 3-hydroxytyramine
5-HIAA : 5-hydroxy-3-indoleacetic acid
HVA : Homovanilic acid (4-hydroxy-3-methoxyphenylacetic acid)
5-HT : 5-hydroxytryptamine (serotonin)
For 1 mg/ml concentration of individual STOCK A solutions:
NE 9.45 mg/ 5 ml of HPLC grade water
DOPAC 5.00 mg/ 5 ml “
DA 5.00 mg/ 4.03 ml “
5-HIAA 5.00 mg/ 5 ml “
HVA 5.00 mg/ 5 ml “
5-HT 5.00 mg/ 2.17 ml “
5-HTOL 5.00 mg/ 5 ml “
For 1 μg/ml concentration of individual STOCK B solutions:
5 μl of STOCK A/ 4995 μl of HPLC-grade water
For working solution:
80 μl of STOCK B/ 4920 μl of HPLC grade water to get a 16 pg/μl concentration
40 μl of STOCK B/ 4960 μl of HPLC grade water to get an 8 pg/μl concentration
20 μl of STOCK B/ 4980 μl of HPLC grade water to get a 4 pg/μl concentration
And so on…
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