RECIPE: Gels for Western immunoblots
Tue, Jan 10 2012 08:02
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| MATERIALS | 8% | 10% | 12.5% | 15% | 17.5% |
| 29:1 Acrylamide | 2.2 mL | 2.7 | 3.3 | 4.0 | 4.67 |
| 4X Lower Tris | 2.0 mL | 2 | 2 | 2 | 2 |
| ddH2O | 3.8 mL | 3.25 | 2.65 | 1.95 | 1.3 |
| APS | 40 uL | 40 uL | 40 uL | 40 uL | 40 uL |
| TEMED | 5 uL | 5 uL | 5 uL | 5 uL | 5 uL |
| | | | | | |
| Total Vol: | 8 mL | 8 mL | 8 mL | 8 mL | 8 mL |
Separation gel:
Stacking gel:
| MATERIALS | |
| 29:1 Acrylamide | 0.7 mL |
| 4X Upper Tris | 1 mL |
| ddH2O | 2.3 mL |
| APS | 20 uL |
| TEMED | 2.5 uL |
| | |
| Total Vol | 4 mL |
Note: These recipes are for single gel, for double gels simply double everything.
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