RECIPE: Cresyl violet staining for mounted slides
Tue, Jan 10 2012 07:47
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Cresyl violet staining for mounted slides.
1. 95% ethanol …………………………… 10 min
2. 70% ethanol …………………………… 1 min
3. 50% ethanol …………………………… 1 min
4. dH2O …………………………… 2 min
5. dH2O …………………………… 1 min
6. Cresyl violet stain*…………………………… 10 min
7. dH2O …………………………… 2 min
8. 50% ethanol …………………………… 1 min
9. 70% acid ethanol+ ………………………… 2 min
10. 95% ethanol …………………………… 2 min
11. 95% ethanol …………………………… 30 seconds
12. 100% ethanol …………………………… 1 min
13. Xylene …………………………… 3 min
14. Air dry and coverslip.
* Cresyl violet stain: add 1.25 g cresyl violet acetate and 0.75 mL glacial acetic acid to 250 mL of warm dH2O, then cool and filter.
+ 70% acid ethanol: 2 mL glacial acetic acid in 200 mL 70% ethanol.
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